FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l04_n01_D3S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l04_n01_D3S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25184095
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC290820.11547764571250227TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG59500.029.755615
CGTCTGA64200.027.78747616
CTCGTAT57000.027.08868642
TCGTATG58100.026.07348843
ATCGGAA70800.025.2642272
TCGGAAG74250.024.150943
CACGTCT77350.023.26691814
CACACGT78200.022.95648412
CGTATGC65400.022.33698544
CAGTCAC85100.020.80576527
ACACGTC94200.019.46327613
GATCGGA96300.018.764591
TCTCGTA86050.018.38836541
AGTCACC96650.018.29652828
CCAGTCA101900.017.46464726
CGGAAGA107150.017.0504554
TCCAGTC107050.016.51900525
GTATGCC97150.015.80149945
GAGCACA117850.015.7292079
AGCACAC120400.015.35870410
Produced by FastQC (version 0.11.5)