FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l04_n01_D3S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l04_n01_D3S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15729322
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1111700.7067691792437081TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG151500.037.95353715
CGTCTGA152650.037.62339816
AGTCACG151050.037.39974628
ATCGGAA156600.037.22622
TCGGAAG159800.036.4665453
CTCGTAT147100.036.3115342
TCGTATG147000.036.01696443
CACGTCT161900.035.7373714
TATCTCG154600.035.72294239
GTCACGC160050.035.43858729
CGTATGC149250.035.2021744
CACACGT164950.035.18567312
GTATGCC152900.034.3762345
GATCGGA172050.034.190171
CGCCAAT164550.033.99142533
ACACGTC171100.033.93411313
CAGTCAC168150.033.7556527
ACGCCAA167400.033.35895532
CACGCCA169600.033.28441631
CGGAAGA177000.033.0245974
Produced by FastQC (version 0.11.5)