FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l04_n01_D3S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l04_n01_D3S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25661862
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC743040.28955030620926886TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG105600.037.5792215
CGTCTGA107900.036.65308416
CTCGTAT106050.035.0828942
TCGTATG110700.034.26051743
ATCGGAA120150.033.5386052
TCGGAAG121650.033.162053
GTCACAC120350.032.30457729
CGTATGC117900.032.1107544
CACGTCT128400.031.18629814
CACACGT129650.030.86826912
CAGTCAC127900.030.48367727
AGTCACA130950.029.602528
ACACGTC144050.028.1573213
TCTCGTA134250.027.98166341
CACAGTG138150.027.75233533
AGTGATC135250.027.6680236
ACACAGT139250.027.61346432
CCAGTCA146400.026.66365626
AGCACAC153550.026.3566510
GATCGGA155150.026.0205571
Produced by FastQC (version 0.11.5)