FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l04_n01_D3S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l04_n01_D3S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22204932
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG31900.019.11114515
CGTCTGA34700.017.89318716
TCGTATG31400.015.84279943
CTCGTAT33500.014.98344742
AGTCACT41050.014.35907728
TCGGAAG43850.014.3153633
ATCGGAA45300.013.7081422
CACGTCT49500.012.99756714
CGTATGC37200.012.94899844
ACTGACC49000.012.0302332
CAGTCAC52550.011.85858227
CACACGT57150.011.33646112
AATCTCG51700.010.96988339
GTCACTG57550.010.320806529
ACACGTC65250.010.13603313
TATAGGC35550.09.80884327
CCAGTCA65950.09.7225526
TCTCGTA55150.09.67285241
ATCGGTG58050.09.572116
GACCAAT62650.09.48109235
Produced by FastQC (version 0.11.5)