FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l04_n01_D3S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l04_n01_D3S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30444076
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG57400.028.14354932
ACGTCTG60650.027.26272815
CGTCTGA64400.025.74508916
CTCGTAT59550.025.39974242
TCGTATG62200.023.84795443
TTAGGCA69300.023.37609534
AGTCACT74050.022.11830928
GTCACTT74500.021.89465129
CGTATGC67800.021.77857844
TCACTTA75250.021.67643230
CTTAGGC76050.021.33087733
CACTTAG77250.021.2030931
ATCGGAA81450.020.7456262
TCGGAAG82600.020.5385133
CACGTCT82150.020.4560414
CACACGT91450.018.5479612
CAGTCAC89400.018.39575427
TCTCGTA92550.016.85377541
TAGGCAT100050.016.28159735
ACACGTC104700.016.26513513
Produced by FastQC (version 0.11.5)