FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l04_n01_D3S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l04_n01_D3S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27298786
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG43250.024.23892815
CGTCTGA46900.022.73626316
TCGTATG42100.021.8677443
CTCGTAT44050.021.15430842
ACCGATG53600.019.47676832
TCGGAAG57250.018.668413
ATCGGAA57750.018.2730082
CGTATGC50100.018.0613544
CACCGAT59650.017.57677531
GATGTAT58450.017.47600735
CACGTCT63600.017.04896414
CACACGT67900.015.86988512
CAGTCAC67650.015.76301327
TATCTCG62550.015.68648239
GTATCTC71450.014.55139138
TCTCGTA70800.013.92465341
AGTCACC79850.013.72128128
ACACGTC82250.013.62076513
TGTATCT81750.012.77228937
CGGAAGA91400.012.3579584
Produced by FastQC (version 0.11.5)