FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l04_n01_D3S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l04_n01_D3S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24965362
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC328040.13139805463265464TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG63350.030.71735215
CTCGTAT57650.029.71200242
CGTCTGA66300.029.38452316
TCGTATG63300.028.6255343
CGTATGC70200.025.71540844
TCGGAAG79500.025.0185453
ATCGGAA80450.024.4435392
CACACGT82650.023.81634512
CACGTCT85300.023.15556514
CAGTCAC85100.022.97331227
GTCACAT84250.022.83234229
AGTCACA87950.022.0247628
TCACGAT87450.021.68886835
TCTCGTA84750.020.47671941
ACACGTC98950.020.02949513
ATCACGA96050.019.8171534
CATCACG101400.019.0822133
CCAGTCA102700.018.97157926
AGCACAC106100.018.65852210
GAGCACA109050.018.339449
Produced by FastQC (version 0.11.5)