FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l03_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l03_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16964184
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGT5150.025.73406237
GTGTAGA5350.024.39658230
GAGTGTA5150.024.03348228
GTAGATC5900.023.62075832
TAGATCT5800.023.59385533
AGTGTAG5300.023.35301229
CACTCTT9800.021.8428972
GTGTAGG7350.018.70858617
TCGTGTA7400.018.58355515
TGTAGGG7550.017.8884918
AGCGGTT6500.016.9852110
TAGGGAA8050.016.77949320
TTCCCTA4700.016.7667588
GTAGGGA8200.016.74749219
TGTAGAT7850.016.33303331
GATCTCG9200.015.58486135
CTCGGTG9200.015.38225938
ATCGGAA12650.013.53741552
TCGGTGG11350.013.06236739
AGATCTC11050.012.97406134
Produced by FastQC (version 0.11.5)