FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l03_n02_A3S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l03_n02_A3S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12396159
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT12850.013.8437932
GTAGAAC16600.012.60368611
GCGTGAC17200.011.13256551
GTACTCG29950.09.9981824
TACTCGA29400.09.87895
GTGACTC19750.09.6899513
CTAGCAC19550.09.6684514
TAGAACA21250.09.42413212
AAAATCC38750.09.3545713
GGTAGAA22950.09.3121810
AGTACTC33350.09.2489933
GGAAAAT49350.09.175161
AATCGAC12900.09.0756955
AAATCCT37500.09.0058814
GCTCTAG21800.08.87492411
TCAGGTA24350.08.6865287
AGTCTCG7700.08.1871655
GAATCGA21000.08.1481784
CACTCGA16200.08.060824
CTATAAG13650.07.91725874
Produced by FastQC (version 0.11.5)