FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l03_n02_A3S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l03_n02_A3S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20965216
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT22050.013.4803062
GTAGAAC30950.011.55754811
GCGTGAC29050.010.8563891
TCAGGTA41150.09.952177
GAATCGA40200.09.8012184
GTGACTC31750.09.50237753
CAGGTAG40600.09.4768628
GTACTCG46350.09.3750944
GGTAGAA40200.09.345610
TACTCGA46500.09.1027575
TAGAACA40400.08.96715312
CTCGAAT21100.08.8613341
GAATTGA53650.08.8548414
AGTACTC50300.08.6837023
AATCGAA40900.08.5325045
GGAAAAT88850.08.5189341
AATTGAC35200.08.5070115
CTCTATA39150.08.50348645
AAAATCC64150.08.2829783
GCTCTAG43000.08.0571511
Produced by FastQC (version 0.11.5)