FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l03_n02_A3S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l03_n02_A3S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25424256
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC44250.010.21913111
CGTGACT23650.09.2359062
TCAGGTA54200.09.1334157
GGAAAAT99200.08.6065641
TAGAACA53550.08.36193612
GAATCGA47600.08.2774974
GGTAGAA55900.08.08908310
CAGGTAG58100.08.0164928
CTCGAAT25100.07.9876411
GTGACTC32750.07.5623243
TACTCGA49800.07.5501265
ATGTCTC93050.07.51809443
GTACTCG47550.07.48125554
AATTGAC40400.07.4677635
GCGTGAC33850.07.45352271
AAAATCC72950.07.4072993
GAATTGA62950.07.40359264
GGTAGGC24700.07.2879087
GGAGAAT74550.07.07083031
AATCGAA47800.07.0652975
Produced by FastQC (version 0.11.5)