FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l03_n02_A3S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l03_n02_A3S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32009690
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG21350.012.75282316
CGTGACT30300.012.3366322
GTAGAAC50500.011.18188511
TCAGGTA61950.09.8430857
TGTAGGG29400.09.72018618
AGTGTAG27600.09.69876229
GAGTGTA28450.09.567245528
GTGTAGG29600.09.35044517
GTACTCG64950.09.2555194
GCGTGAC44400.09.1831391
TCGTGTA30650.09.10353515
GTGACTC43500.09.0578173
GAATCGA55250.09.0059324
TACTCGA66850.08.9587535
GGTAGAA64100.08.91448310
TAGAACA65350.08.64250312
GGAAAAT121450.08.4393571
AAAATCC90550.08.3545983
AATTGAC51950.08.2344655
CTATAAG36350.08.1759494
Produced by FastQC (version 0.11.5)