FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l03_n02_A3S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l03_n02_A3S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24757331
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT24500.013.3273052
GTAGAAC36200.011.24858611
GCGTGAC34400.010.281291
GTGACTC34500.09.9197513
TCAGGTA43750.09.5148777
GGAAAAT97800.09.2826621
GAATCGA43350.09.1929934
GTACTCG58050.08.8431184
TACTCGA59350.08.76322655
CTAGCAC41800.08.66699914
TAGAACA48500.08.490252512
GGTAGAA48900.08.46495310
CTCGAAT25850.08.4531311
GCTCTAG45150.08.27138511
AGTACTC65000.08.2440373
AAAATCC73350.08.13433553
AATCGAA49750.08.100895
CAGGTAG50400.08.0804838
AGTTACG6155.0931703E-108.0519166
TATAAGG28250.07.9699275