FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l03_n02_A3S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l03_n02_A3S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20610409
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC29800.09.05944611
CGTGACT20200.08.9181742
TACTCGA51750.08.70138555
GGAAAAT79400.08.3979661
GTACTCG54100.08.3234144
TATAAGG19800.08.3009235
AGTACTC58750.08.2778553
GAATTGA49850.08.2652244
AATCGAA42400.08.0182545
AATTGAC35950.07.89115145
CTATAAG20600.07.8692624
TCAGGTA39200.07.86404567
AAAATCC59300.07.82139973
AGAACGG125800.07.73173
GACTTAT33850.07.64905745
GAATCGA36600.07.6279774
GGACTTA32500.07.41257674
TAATCGA13700.07.3953834
GGGAAAT42400.07.38502361
GCGTGAC27000.07.3421261
Produced by FastQC (version 0.11.5)