FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l03_n02_A3S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l03_n02_A3S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26124104
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC36250.010.55055511
CGTGACT23550.09.3706482
AATCGAA58650.08.8677185
GAATCGA44500.08.8035354
GTACTCG58950.08.7843964
GCGTGAC29450.08.7199631
TACTCGA61050.08.482235
TCAGGTA50100.08.308997
AGTACTC68350.08.3011023
CAGGTAG50350.08.2671958
GGAAAAT109750.08.251191
GGTAGAA49350.08.20557310
AAAATCC84600.08.170373
TAGAACA47000.08.13889112
GGGAAAT62250.07.67169951
AGTCTCG19750.07.6379285
GAATTGA65000.07.620394
GGGGAAT53050.07.55839731
GGAGAAT72350.07.31685351
ACACGCG26000.07.27204476
Produced by FastQC (version 0.11.5)