FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l03_n02_A3S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l03_n02_A3S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26150613
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT26100.012.6828782
GTAGAAC31700.011.42612111
GCGTGAC33800.010.2638931
GTACTCG61250.09.5941114
TACTCGA62550.09.1067345
TCAGGTA45750.09.0496717
GTGACTC39250.08.9487243
AAAATCC75750.08.6791333
AGTACTC70650.08.3495923
GGAAAAT104900.08.332291
TAGAACA44150.08.25650412
CTAGCAC41650.08.211966514
GGTAGAA46150.08.14067410
CAGGTAG48500.07.97941838
GAATCGA45200.07.8702584
GAATTGA68600.07.8112984
AATTGAC42300.07.7710915
GCTCTAG48250.07.64678211
TGACTCT52050.07.56985664
TATAAGG24400.07.5664745
Produced by FastQC (version 0.11.5)