FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l03_n02_A3S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l03_n02_A3S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34107574
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT34000.011.9877262
GTAGAAC46700.010.93555811
GTACTCG72000.010.2567484
GCGTGAC44350.010.1080071
TACTCGA73700.09.8673855
GGAAAAT157150.09.4609691
AGTACTC84350.09.0487523
AAAATCC112000.09.0261473
TCAGGTA62250.08.9642857
GTGACTC47500.08.6742433
GAATTGA85700.08.5382894
GAATCGA57400.08.4724724
TAGAACA62900.08.33519212
AAATCCT118600.08.1440594
AATCGAA73850.08.0181165
GGTAGAA67100.08.01296610
CTCGAAT34200.07.90425971
AATCCTT97800.07.7121065
CTAGCAC60050.07.681714514
CAGGTAG69650.07.6561228