FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l03_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l03_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16964184
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG7150.030.1605115
CGTCTGA7400.028.83827216
CACGTCT8650.025.19332314
CACACGT9400.023.66122212
ACACGTC10900.021.2295413
AGCACAC11350.019.8007310
CCGAGAC6900.019.61783829
ATCGGAA15850.018.8522172
TCGGAAG15850.018.7104723
AGACCGA7300.018.49077832
GAGACCG7600.017.48256531
ACCGATC7250.017.3668834
AGCGGTT8100.016.92473611
GATCGGA18000.016.8529381
CGGAAGA18150.016.5870154
GCACACG14350.016.1310411
CAGTCAC9100.016.11436727
CCAGTCA9350.015.92582326
GGAATGC9450.015.50537523
CGGTTCA10150.014.16588713
Produced by FastQC (version 0.11.5)