FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l03_n01_A3S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l03_n01_A3S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12396159
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG18250.025.14776215
AGTCACG19600.023.30133228
CGTCTGA20450.022.66240116
CTCGTAT19200.022.15470942
TCGTATG21150.020.53781343
CACGTCT26500.018.08258414
TCGGAAG26250.017.9144443
ATCGGAA26600.017.7633152
GTCACGA26850.017.42849329
CGTATGC25150.017.36056944
CACACGT27800.017.3179212
ACACGTC34550.014.71591113
CAGTCAC35400.013.98136927
TCTCGTA33900.013.34448541
TCACGAT35200.012.65503430
CGGAAGA40550.012.4292094
GATCGGA40300.012.0627261
TCCAGTC40100.011.8945525
CCAGTCA39600.011.760612526
ACGATCA40950.011.75711232
Produced by FastQC (version 0.11.5)