FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l03_n01_A3S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l03_n01_A3S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20965216
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG35400.026.62843915
CGTCTGA38300.024.96462616
CTCGTAT33350.020.58276242
GTCACAC46950.019.9821729
TCGTATG34200.019.74221443
CACACGT50800.019.53029412
TCGGAAG50050.019.5111453
CACGTCT49950.019.50232114
ATCGGAA50650.019.1467442
CGTATGC40650.016.44327544
AGTCACA58050.016.3550728
ACACGTC62400.016.00782213
CAGTCAC64600.015.32358227
CACACTT62900.015.09395131
AATCTCG52600.015.01719739
CCAGTCA67700.013.85811226
CGGAAGA77000.013.5004534
GATCGGA73050.013.4936911
TCACACT70200.013.26799930
TCTCGTA57450.012.81021341
Produced by FastQC (version 0.11.5)