FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l03_n01_A3S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l03_n01_A3S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25424256
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC267680.10528528347102861TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG58150.028.32020615
CGTCTGA60750.027.07116916
CTCGTAT54650.025.57325642
TCGTATG57700.023.8706643
CACGTCT72000.023.27866214
ATCGGAA74150.022.4249972
TCGGAAG75050.022.1860563
CACACGT76050.021.74314512
CGTATGC66450.020.5240144
ACACGTC91650.018.63131513
CAGTCAC91900.018.11568327
GATCGGA96400.017.5091291
AGTCACC97700.017.36292828
TCTCGTA86900.016.85954341
CGGAAGA101900.016.7155554
CCAGTCA98550.016.48318926
TCCAGTC102100.016.0423225
GTATGCC90800.015.51594345
AGCACAC114200.015.01146510
ACTCCAG117850.014.50986923
Produced by FastQC (version 0.11.5)