FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l03_n01_A3S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l03_n01_A3S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32009690
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1549250.48399406554702656TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG218650.038.84242215
AGTCACG218400.037.98085828
CGTCTGA224350.037.8556216
CTCGTAT214750.036.40704742
ATCGGAA240200.036.036812
TCGTATG215500.036.02997643
TCGGAAG241000.035.8985183
CACGTCT240550.035.44627814
GTCACGC235650.035.36289229
CACACGT245900.034.79401812
TATCTCG235300.034.46877339
CGTATGC225950.033.9151944
ACACGTC257450.033.47773413
CACGCCA251100.033.0705631
CGCCAAT247950.033.05578233
ACGCCAA250950.032.60621332
CAGTCAC261250.032.10427
TCACGCC265300.031.61425230
GATCGGA276150.031.597331
CCAGTCA265150.031.39548926
Produced by FastQC (version 0.11.5)