FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l03_n01_A3S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l03_n01_A3S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24757331
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC299810.1210994836236588TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT55100.027.48836942
ACGTCTG59200.027.32395615
TCGTATG57600.026.80366743
CGTCTGA60250.026.58644116
CGTATGC64850.024.15405544
GTCACAC71350.022.00940929
CACACGT75650.021.62030812
CACGTCT77200.021.39021514
TCGGAAG78400.020.9508653
ATCGGAA79850.020.6549512
AGTGATC80400.019.11289636
AGTCACA82200.019.02216328
TCTCGTA84450.018.3613741
ACACGTC92050.018.28159913
CAGTCAC89500.018.09889227
CACAGTG86700.018.035433
ACACAGT87600.017.90092832
CCAGTCA89900.017.6185626
GTATGCC94250.016.8344745
CAGTGAT98200.015.9921135
Produced by FastQC (version 0.11.5)