FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l03_n01_A3S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l03_n01_A3S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20610409
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG38700.025.46248615
CGTCTGA41750.023.71012516
CTCGTAT36350.022.35075642
AGTCACT46300.021.08894328
TCGTATG38950.020.5700443
CACGTCT52200.019.43764914
ACTGACC51900.018.9001532
TCGGAAG55400.018.3987053
ATCGGAA55650.018.2756182
CGTATGC46300.017.35312544
CACACGT60400.016.91046512
AATCTCG54400.016.6296839
ACACGTC67500.015.565021513
CAGTCAC66100.015.24808427
GTCACTG65800.015.07852329
CACTGAC66950.015.0547531
CGGAAGA73800.014.4517614
CCAGTCA68650.014.42011726
GACCAAT65250.014.37859335
TGACCAA70900.013.70863334
Produced by FastQC (version 0.11.5)