FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l03_n01_A3S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l03_n01_A3S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26124104
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG44050.026.60976832
ACGTCTG46200.025.80907215
CGTCTGA47200.025.45292916
CTCGTAT45050.023.52913542
TCGTATG49000.021.2191243
TCGGAAG59900.020.4727973
AGTCACT57650.020.33235528
ATCGGAA61850.019.645432
GTCACTT59950.019.47724229
TTAGGCA61350.019.47328434
TCACTTA62050.019.14438630
CGTATGC54200.019.14163244
CACTTAG62200.019.13438831
CACGTCT64200.019.06341614
CACACGT65550.018.77376712
CTTAGGC67300.017.4841733
ACACGTC79350.015.76393613
CAGTCAC80550.015.194077527
CGGAAGA87400.014.5975214
GTATGCC81200.013.6361145
Produced by FastQC (version 0.11.5)