FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l03_n01_A3S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l03_n01_A3S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26150613
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG43050.024.03927215
CTCGTAT41850.022.53242942
CGTCTGA46450.022.32823216
TCGTATG43150.021.69719743
TCGGAAG59600.018.0077133
ACCGATG58350.017.8905532
CACGTCT60750.017.81289914
ATCGGAA60100.017.5583952
CGTATGC53050.017.35114744
CACCGAT60600.017.15204831
GATGTAT60500.016.88354735
TATCTCG58150.016.8345239
CACACGT64700.016.69063212
GTATCTC70650.014.55588638
ACACGTC78650.013.93047713
CAGTCAC76900.013.691792527
TCTCGTA74200.013.16359241
TGTATCT79300.013.1377237
AGTCACC85250.012.93150728
CGGAAGA88250.012.49303154
Produced by FastQC (version 0.11.5)