FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l03_n01_A3S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l03_n01_A3S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34107574
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG58550.024.78404615
CGTCTGA62000.023.36868316
CTCGTAT54550.022.40198542
TCGTATG60550.021.59473243
CGTATGC70450.018.623844
CACGTCT80150.018.21704914
TCGGAAG80950.018.0950953
CACACGT82650.017.99266412
ATCGGAA83350.017.628052
TCACGAT89200.015.941009535
GTCACAT90050.015.8648929
ACACGTC100250.015.10314913
AGTCACA96050.014.92069628
CAGTCAC105650.014.26743827
ATCACGA105200.013.83730534
TCTCGTA91000.013.70092241
CCAGTCA107450.013.44277826
AGCACAC115550.013.14227910
GATCGGA115350.012.9355711
CATCACG114100.012.83675233
Produced by FastQC (version 0.11.5)