FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l02_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l02_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16733426
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT8500.021.2037012
AGTGTAG4700.019.15486729
TCTCGGT5200.018.57788737
GTAGATC5000.018.42827232
GAGTGTA5150.016.60708628
GTGTAGA5350.016.40290330
TAGATCT6000.016.08088933
ATCGGAA13000.014.5571572
TCGGAAG13050.014.15385153
TGTAGAT6800.013.56079131
TTCCCTA6000.013.1296598
GTGTAGG7250.013.05576516
GATCGGA15050.013.0234091
TCGTGTA7600.012.75066414
TGTAGGG7350.012.57109517
GTAGGGA7550.012.23108719
GATCTCG9500.011.78895535
GTAGAAC4450.011.64690211
CTCGGTG11200.011.63463938
TACTCGA4900.011.4921735
Produced by FastQC (version 0.11.5)