FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l02_n02_N3S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l02_n02_N3S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24712881
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC38400.010.89717811
GAATCGA38550.010.1623244
TCAGGTA46950.09.6321567
CGTGACT20900.09.4818552
GGAAAAT109850.09.4545681
AATCGAA49150.09.2990955
AAAATCC75100.09.2041043
CTCGAAT23800.08.9926461
CAGGTAG50400.08.7492728
CACTCGT37300.08.74487735
TAGAACA48300.08.66415612
GGTAGAA49000.08.63155110
CTCTATA39350.08.51810845
TCACTCG39000.08.47905234
ACTCGTG40250.08.27161636
GAATTGA64100.08.2542574
GGGAAAT51650.07.8513261
AAATCCT84850.07.82777454
GCGGAAA53850.07.69791271
GATCTAG40250.07.6634245