FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l02_n02_N3S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l02_n02_N3S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19070433
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC35800.011.24879211
TCAGGTA42850.010.1337927
CAGGTAG42950.09.7430978
GAATCGA31900.09.7402354
GGTAGAA42550.09.62266210
CTCGAAT19050.09.1064051
AATCGAA39000.08.9484575
TAGAACA46400.08.87357512
GGAAAAT86900.08.7110551
AATCGAC22950.08.142855
AAAATCC67350.07.9901313
AAATCCT73300.07.89423854
AGGTAGA53800.07.6524649
CGTGACT12400.07.6275692
ACGGTAC11350.07.532902220
AGATCAG74450.07.4698754
GTACTCG31400.07.38564834
AATCCTT56800.07.33338455
GAATTGA46850.07.25685454
ACAATCG18200.07.1754673
Produced by FastQC (version 0.11.5)