FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l02_n02_N3S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l02_n02_N3S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26855473
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC43000.011.40575611
TCAGGTA53000.010.1457647
CGTGACT21950.09.5411582
TAGAACA52950.09.22050812
CAGGTAG54900.08.8517438
GGAAAAT104700.08.8005171
GGTAGAA55400.08.77139510
GAATCGA47500.08.6741474
CACTCGT33750.08.6649835
GTACTCG42200.08.1096184
GCGGAAA52250.07.76100251
AATCGAC32350.07.65574935
GAATTGA71850.07.64596564
AATCGAA46900.07.58496435
AAAATCC74400.07.56560473
CTCTATA41700.07.55258645
GGGAAAT62300.07.4130581
CGGGAAT19450.07.41296531
TCACTCG40200.07.330690434
GCGTGAC34000.07.222361
Produced by FastQC (version 0.11.5)