FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l02_n02_N3S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l02_n02_N3S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34520367
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG29100.017.47400516
TGTAGGG37750.013.47002818
GTGTAGG38900.013.07181217
TCGTGTA39350.013.03660715
GAGTGTA36800.012.71503728
AGTGTAG38850.012.10200729
GTAGATC43000.011.50956432
ATCGGAA49750.010.7712663
GTGTAGA46250.010.40888430
GTAGAAC55550.010.36785311
TAGATCT49000.010.1920533
GAATCGA60200.09.9858354
CGTGACT27950.09.8295842
TAGGGAA51450.09.75193520
ATCTCGG56300.09.589643536
CACTCGT51200.09.57824935
GTAGGGA51600.09.46178719
TCTCGGT51400.09.40968237
TCGGAAG64500.08.97104554
TCAGGTA69300.08.9606517
Produced by FastQC (version 0.11.5)