FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l02_n02_N3S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l02_n02_N3S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32351443
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC55850.012.40679711
CGTGACT26250.012.0959792
TCAGGTA65950.011.0874827
GGTAGAA67800.010.35263710
GAATCGA55200.010.1154024
TAGAACA69800.09.86333212
CAGGTAG74500.09.5730718
AAAATCC100400.09.3964913
GGAAAAT140350.09.2297821
GCGTGAC38300.08.8232481
AAATCCT111700.08.5464014
AATCGAC35850.08.3528195
AATCGAA59050.08.2357745
GAATTGA77050.08.1819344
CTCGAAT33600.08.1800621
AGATCAG118950.08.1201384
GGTAGGC31900.07.8993577
AAAAATC95550.07.7066962
GTACTCG58350.07.6786134
AATTGAC48400.07.5825075
Produced by FastQC (version 0.11.5)