FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l02_n02_N3S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l02_n02_N3S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33078148
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC58450.012.23980611
TCAGGTA67700.011.365847
CGTGACT27150.010.6995522
TAGAACA71350.010.059031512
CAGGTAG75100.09.8860548
GGTAGAA73450.09.74003810
GGAAAAT149350.09.1260291
AAAATCC111100.08.9981153
CTCGAAT30800.08.8504711
AATCGAA59100.08.5335685
AGATCAG127500.08.4585414
GAATCGA52350.08.1715984
GTACTCG65450.08.1184394
AAATCCT121500.08.1164864
AATTGAC50950.08.0426175
GAATTGA85000.08.0259024
GGTAGGC34800.08.0169017
GCGTGAC39950.07.95122531
GGGAAAT69100.07.824651
TACTCGA69100.07.81993875
Produced by FastQC (version 0.11.5)