FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l02_n02_N3S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l02_n02_N3S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31946718
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT25200.011.34900952
GTAGAAC49200.011.02010511
TCAGGTA64050.09.6600047
GAATCGA52750.09.4327484
GGAAAAT145950.09.1535841
CACTCGT51250.09.12988335
GAATTGA85350.08.9689944
GCGTGAC37850.08.8091991
CAGGTAG64150.08.7678028
GGTAGAA65650.08.56697610
TAGAACA62650.08.51117712
AAAATCC92500.08.30030253
TCACTCG55350.08.29102634
ACTCGTG57500.08.17662736
CTCTATA59350.08.07339145
AATCGAA61500.07.9808615
CTCGAAT30100.07.858931
GCGGAAA70450.07.7068451
AATTGAC48050.07.68455655
GTACTCG57450.07.64210464
Produced by FastQC (version 0.11.5)