FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l02_n02_N3S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l02_n02_N3S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29816795
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT28350.014.2187262
GTAGAAC45300.011.670814511
GCGTGAC39150.011.2212561
TCAGGTA54050.010.4899177
CTCTATA56200.010.48730145
GGAAAAT132050.09.9123441
CACTCGT56150.09.89557135
AAAATCC92600.09.774773
TCACTCG59800.09.517334
ACTCGTG61700.09.47942136
GTGACTC49150.09.2537833
GGTAGAA59100.09.21197710
GAATCGA43800.09.2015644
GAATTGA72250.09.1932034
GCGGAAA78650.09.0229891
TAGAACA58900.08.93845912
GTACTCG57200.08.8172824
CTAGCAC50950.08.78765914
CAGGTAG64450.08.7270518
GCTCTAG55800.08.4264311
Produced by FastQC (version 0.11.5)