FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l02_n02_N3S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l02_n02_N3S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30442922
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT28600.013.2282572
GTAGAAC48650.011.69954111
TCAGGTA57150.010.4721147
GCGTGAC39700.010.4418131
AAAATCC94500.09.7687893
GGTAGAA61450.09.44540110
TAGAACA60650.09.42242212
GGAAAAT143350.09.3669281
CAGGTAG65250.08.9304038
GAATTGA79300.08.7732054
GAATCGA47650.08.5524134
GTGACTC49700.08.4717653
AACGGAC23150.08.3641235
AATCGAA60050.08.28613955
AAATCCT106200.08.2682394
CACTCGT58250.08.18724135
GTACTCG59150.08.1838314
TACTCGA61450.08.1706385
CTCGAAT26200.07.9970591
AATTGAC47050.07.9436745
Produced by FastQC (version 0.11.5)