FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l02_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l02_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16733426
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG11050.032.72105415
CGTCTGA11400.031.9142216
CACGTCT13400.027.15147414
CACACGT13500.026.7839912
ACACGTC14650.025.29469913
CAGTCAC10700.023.4104827
ATCGGAA19350.023.2158932
TCGGAAG19400.023.040283
AGCACAC17250.022.00596810
CCAGTCA13150.020.93655626
GATCGGA22450.020.0143241
GCACACG19250.019.95300311
CGGAAGA23600.019.5109424
CTCCAGT19700.017.41225624
AACTCCA22450.016.77468922
AGTCACT4700.013.924149528
AGAGCAC27700.013.8658848
CCGAGAC6100.012.94808229
AGACCGA6100.012.9090532
GAACTCC30150.012.41059921
Produced by FastQC (version 0.11.5)