FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l02_n01_N3S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l02_n01_N3S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24712881
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG34650.025.12741328
ACGTCTG36650.024.30877315
CGTCTGA38200.023.0283216
CTCGTAT36350.021.54575242
TCGTATG40850.019.6133543
GTCACGA49650.017.85334829
CGTATGC45800.017.29676444
TCGGAAG52400.016.9623783
ATCGGAA52800.016.8764922
CACACGT58200.016.08080512
CACGTCT57700.015.86936614
CAGTCAC60300.015.29707427
TCACGAT58150.014.97283130
ACACGTC69800.013.92405613
TCTCGTA61250.013.301141
CCAGTCA75000.011.99889926
AGCACAC85550.011.334310
GATCGGA82050.011.1639651
ACGATCA80900.011.15167932
GAGCACA88900.011.0084189
Produced by FastQC (version 0.11.5)