FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l02_n01_N3S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l02_n01_N3S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19070433
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG27550.023.19166215
CGTCTGA28800.021.79529816
TCGGAAG37050.017.0053143
ATCGGAA37100.016.9823972
GTCACAC37000.016.35632329
CTCGTAT22150.015.95249142
CACACGT40350.015.89037712
CACGTCT44100.014.74325114
CAGTCAC45800.013.90139527
TATGGGT23750.013.0774743
TCGTATG26050.013.04600343
ACACGTC52050.012.96681713
AGTCACA48400.012.78266328
CACACTT51850.011.80205331
GATCGGA58500.011.1568131
CCAGTCA57350.010.86635226
CGGAAGA63950.010.6966534
TATAGGC38000.010.65678927
ATATGGG30200.010.50787142
TCACACT60900.010.417582530
Produced by FastQC (version 0.11.5)