FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l02_n01_N3S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l02_n01_N3S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26855473
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC275950.10275372919330075TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG66000.029.21279515
CGTCTGA68050.028.0359516
CTCGTAT58250.025.69371642
TCGTATG59450.024.53165243
CACACGT79750.024.1759712
ATCGGAA80700.023.9238382
CACGTCT82900.023.42021614
TCGGAAG83700.023.2007733
ACACGTC98600.019.94198213
CAGTCAC94750.019.8979527
CGTATGC72350.019.4416844
GATCGGA110000.017.6974581
AGTCACC106700.017.6903428
CCAGTCA108450.017.4673126
TCTCGTA88000.017.33990141
CGGAAGA116250.017.0336134
TCCAGTC114300.016.71125425
GCACACG126250.015.84178711
AGCACAC129450.015.48493810
ACTCCAG130400.015.30365623
Produced by FastQC (version 0.11.5)