FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l02_n01_N3S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l02_n01_N3S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34520367
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC2430590.7041031748011254TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG365500.039.49875615
AGTCACG359000.039.13585728
CGTCTGA369350.039.0577116
CTCGTAT331900.037.92592242
ATCGGAA381450.037.8657462
TCGGAAG382550.037.7509843
GTCACGC376650.037.46917729
CACGTCT387450.037.3828314
TCGTATG331400.037.2499143
CACACGT391450.037.07544312
TATCTCG362350.036.9347839
CAGTCAC396250.035.5651327
CACGCCA394550.035.48442531
ACACGTC412000.035.36814513
ACGCCAA391300.035.35373332
CGCCAAT393300.035.2090433
CGTATGC344350.035.09053844
GATCGGA415400.034.978711
TCACGCC414200.034.2841830
GCCAATA400900.034.19373334
Produced by FastQC (version 0.11.5)