FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l02_n01_N3S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l02_n01_N3S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32351443
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG59250.027.07335915
CGTCTGA62850.025.4874916
TCGTATG61800.024.6906543
CTCGTAT60250.024.69060342
GTCACAC75250.020.65924829
ATCGGAA80750.020.2585642
CGTATGC76250.019.95219844
TCGGAAG84300.019.779143
CACACGT83550.019.71070712
CACGTCT87400.018.71382714
CAGTCAC91400.017.7227327
AGTCACA92950.016.84620528
TCTCGTA96300.015.93839941
ACACGTC108350.015.59367913
AGTGATC99250.015.029345536
CCAGTCA108350.014.86721926
CACAGTG106100.014.54656433
GATCGGA115950.014.34433651
ACACAGT109450.014.18333332
TCCAGTC116900.013.97242525
Produced by FastQC (version 0.11.5)