FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l02_n01_N3S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l02_n01_N3S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33078148
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG59450.023.00867715
CGTCTGA63350.021.48614716
CTCGTAT49250.019.51260242
TCGTATG51800.018.55218143
AGTCACT71900.018.36746228
ATCGGAA79650.017.3178022
TCGGAAG83500.016.8965933
ACTGACC78600.016.60162532
CACGTCT85350.016.50096914
CACACGT85200.016.3715612
CAGTCAC89650.014.98193527
CGTATGC61850.014.7005444
ACACGTC104550.013.70734513
GTCACTG97700.013.42504929
CACTGAC101050.013.3362931
AATCTCG92450.012.53607939
CCAGTCA109800.012.37594926
TCTCGTA83050.012.35712541
TGACCAA109850.012.10443234
GATCGGA117600.011.9800681
Produced by FastQC (version 0.11.5)