FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l02_n01_N3S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l02_n01_N3S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31946718
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG48850.026.85020332
ACGTCTG50700.026.71324715
CGTCTGA54400.025.10356516
CTCGTAT50100.023.00001342
TCGTATG51900.021.98564343
CTTAGGC60500.021.75482733
TCACTTA63450.020.81357430
TTAGGCA64050.020.7600134
AGTCACT66500.020.09562928
GTCACTT67300.019.75661729
CACTTAG69150.019.0329731
TCGGAAG74500.018.6661173
ATCGGAA75200.018.4025922
CACACGT76700.018.21510312
CACGTCT76500.017.99810414
CGTATGC64950.017.5679344
CAGTCAC85250.016.12457827
ACACGTC92150.015.47851613
TAGGCAT88300.015.26253535
TCTCGTA87250.013.80011941
Produced by FastQC (version 0.11.5)