FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l02_n01_N3S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l02_n01_N3S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29816795
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG60300.029.58656115
CGTCTGA62200.028.75604816
CTCGTAT55650.026.93421442
TCGTATG57350.025.19419543
ACCGATG70700.024.34365532
ATCGGAA80400.022.4180642
CACCGAT76800.022.38073731
TCGGAAG81450.022.2395733
CACACGT84400.021.83121512
CGTATGC64950.021.6567844
CACGTCT84950.021.61049314
TATCTCG75900.021.43707539
GATGTAT81150.021.18159935
CAGTCAC87450.020.22104327
GTATCTC90850.018.7757838
TCTCGTA83000.018.51980441
ACACGTC103250.018.25956313
AGTCACC103000.017.34290728
TGTATCT104600.016.60794837
CCAGTCA107850.016.41708826
Produced by FastQC (version 0.11.5)