FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l02_n01_N3S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l02_n01_N3S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30442922
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG52350.026.3442915
CGTCTGA54100.025.32623316
CTCGTAT49000.022.91914642
TCGTATG53700.021.96119543
TCGGAAG72100.019.3809013
ATCGGAA71800.019.2425022
CACGTCT74900.019.1337314
CGTATGC61100.019.00645344
CACACGT75500.018.53454412
TCACGAT80200.016.46734435
CAGTCAC85550.016.30473327
ACACGTC97850.014.99081413
GTCACAT92700.014.68307229
AGTCACA94200.014.64028328
TCTCGTA79150.014.33092841
ATCACGA100500.013.65593734
CCAGTCA100350.013.47410126
AGCACAC108050.013.42991910
CATCACG103900.013.42553833
GATCGGA108500.013.0469761
Produced by FastQC (version 0.11.5)