FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16406146
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTA3950.019.93396828
CACTCTT9150.019.2109892
GTAGATC4900.016.97069732
TAGATCT5100.016.27928533
AGTGTAG4700.016.27435729
TCTCGGT5200.015.5425537
ATCGGAA11050.014.8880142
TCGGAAG11300.014.3573033
GTGTAGG6950.013.62656117
GTGTAGA5950.013.61140530
TCGTGTA7100.013.0217315
TTCCCTA5500.012.692348
CGGAAGA13650.012.050614
GTAGGGA7200.011.25957619
GATCGGA14150.011.1522021
TGTAGGG8400.010.9893318
TGTAGAT6800.010.91651231
TAGGGAA7450.010.88183720
TCCCTAC5600.010.8532149
GATCTCG9150.010.76820535
Produced by FastQC (version 0.11.5)