FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l01_n02_P3S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l01_n02_P3S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25056350
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT22350.012.7945822
GTAGAAC34950.011.26530811
GCGTGAC32650.09.9353091
TCAGGTA45450.09.4558437
GTACTCG51650.09.3276694
GAATCGA40350.09.0943944
GGAAAAT105300.08.9423151
TACTCGA54500.08.8811995
CTCGAAT25150.08.8674611
AAAATCC75050.08.5491683
GGTAGAA49100.08.52279410
CAGGTAG49400.08.4259818
AGTACTC63200.08.4066823
TAGAACA46850.08.35617412
AATCGAA50700.08.2591375
AATCGAG35200.07.93064835
GTGACTC40050.07.86964563
CTAGCAC41850.07.741704514
GAATTGA67500.07.70438344
AGATCAG90850.07.4588394
Produced by FastQC (version 0.11.5)