FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l01_n02_P3S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l01_n02_P3S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24827192
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT21850.013.7056742
GTAGAAC40650.013.28322511
TCAGGTA50400.011.25057
GCGTGAC31100.011.0823921
GGTAGAA51950.010.61041810
CAGGTAG52800.010.4402378
TAGAACA53550.010.29372412
GAATCGA41800.09.9100164
AAAATCC74450.09.4345833
GGAAAAT105000.09.2253241
CGTTACG3206.944716E-69.13920943
GTGACTC39000.08.7742863
AGGTAGA63300.08.6726039
AATCGAA49500.08.5958625
AGATCAG95350.08.358254
AAATCCT79150.08.305484
GTACTCG48750.08.220124
CTAGCAC39400.08.16593214
CTCGAAT25400.07.8933031
TACTCGA49500.07.77720835
Produced by FastQC (version 0.11.5)